221198
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221198
Sigma-Aldrich
Formamide
ACS reagent, ≥99.5%
| Synonym: | Amide C1, Formic amide |
| CAS Number: | 75-12-7 |
| Linear Formula: | HCONH2 |
| Molecular Weight: | 45.04 |
| Beilstein Registry Number: | 505995 |
| EC Number: | 200-842-0 |
| MDL number: | MFCD00007941 |
| PubChem Substance ID: | 24853220 |
Description
| Application | Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures. |
| Packaging | 1, 6×1, 4×4 L in glass btl |
| 100, 500 mL in glass btl | |
| 20 L in ss drum |
Properties
| grade | ACS reagent |
| vapor density | 1.55 (vs air) |
| vapor pressure | 0.08 mmHg ( 20 °C) |
| 30 mmHg ( 129 °C) | |
| assay | ≥99.5% |
| autoignition temp. | 932 °F |
| color | ≤10(APHA) |
| refractive index | n |
| bp | 210 °C(lit.) |
| mp | 2-3 °C(lit.) |
| transition temp | freezing point 2.0-3.0 °C |
| density | 1.134 g/mL at 25 °C(lit.) |
Safety
| Personal Protective Equipment | Eyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| WGK Germany | 1 |
| RTECS | LQ0525000 |
| UEL | 19.00%(V) |
| LEL | 2.70%(V) |
| Flash Point(F) | 302 °F |
| Flash Point(C) | 150 °C |
References
| reference | Casey, J. and Davidson, N., Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 4, 1539, (1977) |
| , Sambrook, J., et al. Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY , (1989), 9.47 | |
| , Ausubel, F.G. et al., ed Curr. Protoc. Mol. Biol. New York , (1999), 2.10.11-2.10.12 | |
| Merck | Merck 13,4261 |
| Beilstein | Beil. 2,IV,45 |
| Fieser | Fieser 1,402 / Fieser 2,201 / Fieser 3,147 |
| Aldrich MSDS 1, 979:B / Corp MSDS 1 (1), 1734:B / FT-IR 1 (1), 747:A / FT-IR 2 (1), 1254:A / IR-Spectra (3), 437:A / IR-Spectra (2), 387:A / NMR-Reference 2 (1), 629:A / RegBook 1 (1), 877:A / Sax 6, 1452 / Structure Index 1, 134:A:1 / Vapor Phase 3, 777:A |
